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Patient Daily | Apr 19, 2026

Study finds cola alters oral bacteria and pH in clear aligner users within hours

A recent study published in the Polish Journal of Microbiology reports that drinking cola can quickly lower pH levels and shift the bacterial balance inside orthodontic clear aligners, with effects observed within just a few hours. The findings were based on a 24-hour microbiome analysis involving 24 healthy female graduate students using custom-made passive clear aligners.

This research matters because it addresses how popular beverages like cola may affect dental health for people undergoing orthodontic treatment with clear aligners. While carbonated drinks are already known to contribute to dental caries, their specific interaction with the microenvironment beneath clear aligners has not been well studied until now.

Researchers used high-throughput genetic sequencing to track changes in both pH and bacterial composition at several time points after participants consumed cola. The results showed that soft drink consumption led to an immediate drop in pH and caused short-term microbial imbalances. These disruptions were most pronounced when participants removed their aligners before drinking, particularly around twelve hours after exposure.

The study found that wearing the aligner during soda consumption might provide some shielding effect against immediate acidity, but this mechanism remains hypothetical according to the authors. In contrast, removing the aligner before drinking allowed more soda residue to remain on tooth surfaces, which could later become trapped when the appliance was replaced—potentially worsening bacterial activity and acid production throughout the day.

Further analysis revealed shifts in specific types of bacteria depending on whether or not subjects wore their aligners while drinking. For example, those who removed their appliances experienced greater increases in Fusobacteria abundance at certain time points compared to those who kept them in place.

The authors conclude that these findings highlight potential risks associated with consuming acidic beverages during orthodontic treatment with clear aligners. They suggest further research is needed to better understand these interactions and inform future oral health recommendations.

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